Long Non-coding RNA TMEM220-AS1 Suppressed Hepatocellular Carcinoma by Regulating the miR-484/MAGI1 Axis as a Competing Endogenous RNA

Long non-coding RNAs (lncRNAs) have a considerable regulatory influence on multiple biological processes. Nevertheless, the role of TMEM220-AS1 in hepatocellular carcinoma (HCC) remains unclear. We used The Cancer Genome Atlas (TCGA) database to analyze differentially expressed lncRNAs. qRT-PCR was verify results for large population. vitro effects HCC cells were determined using Cell Counting Kit-8 (CCK-8), 5-ethynyl-2’-deoxyuridine (EdU), flow cytometry, and Transwell assays cells. western blotting identify epithelial-mesenchymal transition (EMT). Moreover, we performed bioinformatics analysis, blotting, dual luciferase reporter gene assay, RNA pull-down, binding protein immunoprecipitation (RIP) investigate underlying molecular mechanisms function. Finally, function verified vivo . showed that at considerably low levels HCC. It demonstrated malignant phenotypes EMT promoted by knock down both TMEM220-AS1, which detected primarily cytoplasm, functioned as an miRNA sponge bind miR-484 promote level membrane-associated guanylate kinase, WW, PDZ domain containing 1 ( MAGI1 ), thereby curbing In conclusion, proliferation metastasis through miR-484/ axis